Journal: bioRxiv

Article Title: jouvence , a new small nucleolar RNA required in the gut extends lifespan in Drosophila

doi: 10.1101/441121

Figure Lengend Snippet: A) RNA-Seq analysis performed on total-RNA from the gut between the Wilt-Type CS and the deletion reveals that 376 genes are upregulated (A), while 352 are downregulated (B), in deletion compared to Control-CS (Table-S3 and S4 for the complete list of genes). Highlighted in yellow are the selected genes use for validation by RT-qPCR. C&D): Gene Ontology (GO) analysis of the deregulated genes performed with PANTHER classification system . In brief, molecular function selection reveals that the main genes could be classified in catalytic activity, and so both for the up- or down-regulated genes. Selecting for the biological process, the majority of the genes could be grouped into two categories: the cellular and the metabolic processes.

Article Snippet: Mouse and human total RNA were commercially purchased at Amsbio: Mouse Brain, whole Total RNA: MR-201, Mouse Ovary Total RNA: MR-406, Mouse Kidney Total RNA: MR-901, Human Brain Total RNA: HR-201, Human Ovary Total RNA: HR-406, Human Kidney Total RNA: HR-901.

Techniques: RNA Sequencing Assay, Quantitative RT-PCR, Selection, Activity Assay

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Isolated Langerhans cell histiocytosis of the stomach: a case report and literature review

doi:

Figure Lengend Snippet: Immunohistochemical staining for biomarkers in histiocytoid cells. A. Strongly positive staining for S-100 (IHC × 100); B. Strongly positive staining for CD1a (IHC × 100). C. Strongly positive staining for langerin (CD207) (IHC × 100); D. Negative staining for CD21 (IHC × 100); E. Negative staining for BRAF V600E (IHC × 100).

Article Snippet: We used the the following primary antibodies: Vimentin (clone V9, OriGene), S100 (clone poly, OriGene), CD1a (clone O10, OriGene), langerin (clone343828, OriGene), Pan-cytokeratin (clone AE1, OriGene), CD68 (clone PG-M1, OriGene), CD163 (clone 10D6, OriGene), CD21 (clone 2G9, OriGene), CD35 (clone E11, OriGene), CD117 (clone poly, OriGene), CD34 (clone QBEnd-10, OriGene), DOG-1 (clone SP31, OriGene), cyclinD1 (clone DCS-6, OriGene), MLH1 (clone ES05, OriGene), MSH2 (clone 25D12, OriGene), MSH6 (clone EP49, OriGene), PMS2 (clone EP51, OriGene), HP, P53 (clone DO7, OriGene), Ki-67 (clone UMAB107, Ori-Gene).

Techniques: Immunohistochemical staining, Staining, Immunohistochemistry, Negative Staining

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Isolated Langerhans cell histiocytosis of the stomach: a case report and literature review

doi:

Figure Lengend Snippet: Reported cases of adult LCH

Article Snippet: We used the the following primary antibodies: Vimentin (clone V9, OriGene), S100 (clone poly, OriGene), CD1a (clone O10, OriGene), langerin (clone343828, OriGene), Pan-cytokeratin (clone AE1, OriGene), CD68 (clone PG-M1, OriGene), CD163 (clone 10D6, OriGene), CD21 (clone 2G9, OriGene), CD35 (clone E11, OriGene), CD117 (clone poly, OriGene), CD34 (clone QBEnd-10, OriGene), DOG-1 (clone SP31, OriGene), cyclinD1 (clone DCS-6, OriGene), MLH1 (clone ES05, OriGene), MSH2 (clone 25D12, OriGene), MSH6 (clone EP49, OriGene), PMS2 (clone EP51, OriGene), HP, P53 (clone DO7, OriGene), Ki-67 (clone UMAB107, Ori-Gene).

Techniques: Mutagenesis

Journal: Journal of thrombosis and thrombolysis

Article Title: Metal ion chelation enhances tissue plasminogen activator (tPA)-induced thrombolysis: an in vitro and in vivo study.

doi: 10.1007/s11239-021-02600-6

Figure Lengend Snippet: Fig. 5 Photothrombosis and thrombolysis in vivo in the mouse femo- ral artery. A The femoral artery (shown by an arrow) of CD1 mouse. Scale bar: 1 cm. B(1–3) Photothrombotic occlusion of the femoral artery. B-1 Clot indicated by an arrow occluded the femoral artery downstream (outlined by dash lines) of the thrombus formation. The artery occlusion created a blood-free field. B-2. Reperfusion of the femoral artery by the treatment of tPA. B-3 Darker colored clot in a non-reperfused femoral artery. The image (B-3) was taken 30 min after establishing photothrombotic clot. Scale bar for B: 1 mm. C Change of color intensity of clot (thrombus) in non-reperfused femo- ral artery over time. Images of the thrombus were taken every 5 min and was analyzed by ImagePro software. Change of thrombus color intensity was expressed as percentage increase and calculated accord- ing to the formula (F -F0)/F0· %. Values are mean ± SEM. n = 7–9

Article Snippet: Citrated blood of male CD1 mice was purchased from Innovative Research (Novi, MI) and BioChemed Services (Winchester, VA).

Techniques: In Vivo, Software